2× hybridization buffer Search Results


probe mixture (probe/hybridization buffer/purified h 2 o=1:7:2)  (Beijing GP Medical Technologies)
90
Beijing GP Medical Technologies probe mixture (probe/hybridization buffer/purified h 2 o=1:7:2)
Probe Mixture (Probe/Hybridization Buffer/Purified H 2 O=1:7:2), supplied by Beijing GP Medical Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/probe mixture (probe/hybridization buffer/purified h 2 o=1:7:2)/product/Beijing GP Medical Technologies
Average 90 stars, based on 1 article reviews
probe mixture (probe/hybridization buffer/purified h 2 o=1:7:2) - by Bioz Stars, 2026-04
90/100 stars
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hybridization buffer (50% [v/v] formamide, 2× ssc, 10% [v/v] dextran sulfate)  (Progen Biotechnik)
90
Progen Biotechnik hybridization buffer (50% [v/v] formamide, 2× ssc, 10% [v/v] dextran sulfate)
Hybridization Buffer (50% [V/V] Formamide, 2× Ssc, 10% [V/V] Dextran Sulfate), supplied by Progen Biotechnik, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hybridization buffer (50% [v/v] formamide, 2× ssc, 10% [v/v] dextran sulfate)/product/Progen Biotechnik
Average 90 stars, based on 1 article reviews
hybridization buffer (50% [v/v] formamide, 2× ssc, 10% [v/v] dextran sulfate) - by Bioz Stars, 2026-04
90/100 stars
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version 2 hybridization buffer  (Amersham Pharmacia Biotech Ltd)
90
Amersham Pharmacia Biotech Ltd version 2 hybridization buffer
Version 2 Hybridization Buffer, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/version 2 hybridization buffer/product/Amersham Pharmacia Biotech Ltd
Average 90 stars, based on 1 article reviews
version 2 hybridization buffer - by Bioz Stars, 2026-04
90/100 stars
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hybridization buffer (70% deionized formamide, 14.25mm tris-hcl ph 7.2, 1.4% bsa and 0.2m nacl)  (PANAGENE Inc)
90
PANAGENE Inc hybridization buffer (70% deionized formamide, 14.25mm tris-hcl ph 7.2, 1.4% bsa and 0.2m nacl)
Hybridization Buffer (70% Deionized Formamide, 14.25mm Tris Hcl Ph 7.2, 1.4% Bsa And 0.2m Nacl), supplied by PANAGENE Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hybridization buffer (70% deionized formamide, 14.25mm tris-hcl ph 7.2, 1.4% bsa and 0.2m nacl)/product/PANAGENE Inc
Average 90 stars, based on 1 article reviews
hybridization buffer (70% deionized formamide, 14.25mm tris-hcl ph 7.2, 1.4% bsa and 0.2m nacl) - by Bioz Stars, 2026-04
90/100 stars
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spectral karyotyping with fluorescence in situ hybridization (skyfish) chromosome painting techniques  (Specim Spectral Imaging)
90
Specim Spectral Imaging spectral karyotyping with fluorescence in situ hybridization (skyfish) chromosome painting techniques
Spectral Karyotyping With Fluorescence In Situ Hybridization (Skyfish) Chromosome Painting Techniques, supplied by Specim Spectral Imaging, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/spectral karyotyping with fluorescence in situ hybridization (skyfish) chromosome painting techniques/product/Specim Spectral Imaging
Average 90 stars, based on 1 article reviews
spectral karyotyping with fluorescence in situ hybridization (skyfish) chromosome painting techniques - by Bioz Stars, 2026-04
90/100 stars
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hybridization buffer (0.25 m na 2 hpo 4 , ph 7.2; 7% w/v sds; 1 mm edta; and 1% w/v bsa)  (SciGene Inc)
90
SciGene Inc hybridization buffer (0.25 m na 2 hpo 4 , ph 7.2; 7% w/v sds; 1 mm edta; and 1% w/v bsa)
Hybridization Buffer (0.25 M Na 2 Hpo 4 , Ph 7.2; 7% W/V Sds; 1 Mm Edta; And 1% W/V Bsa), supplied by SciGene Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hybridization buffer (0.25 m na 2 hpo 4 , ph 7.2; 7% w/v sds; 1 mm edta; and 1% w/v bsa)/product/SciGene Inc
Average 90 stars, based on 1 article reviews
hybridization buffer (0.25 m na 2 hpo 4 , ph 7.2; 7% w/v sds; 1 mm edta; and 1% w/v bsa) - by Bioz Stars, 2026-04
90/100 stars
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2× hybridization buffer  (Genosensor)
90
Genosensor 2× hybridization buffer
2× Hybridization Buffer, supplied by Genosensor, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/2× hybridization buffer/product/Genosensor
Average 90 stars, based on 1 article reviews
2× hybridization buffer - by Bioz Stars, 2026-04
90/100 stars
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version 2 microarray hybridization buffer  (Amersham Pharmacia Biotech Ltd)
90
Amersham Pharmacia Biotech Ltd version 2 microarray hybridization buffer
Version 2 Microarray Hybridization Buffer, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/version 2 microarray hybridization buffer/product/Amersham Pharmacia Biotech Ltd
Average 90 stars, based on 1 article reviews
version 2 microarray hybridization buffer - by Bioz Stars, 2026-04
90/100 stars
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2× hybridization buffer helicos rna sequencing kit  (Helicos Inc)
90
Helicos Inc 2× hybridization buffer helicos rna sequencing kit
DRS sample preparation. RNA species which contain a 3' poly-A tail require 3' end blocking as described. Other RNA species are enzymatically 3' polyadenylated and 3' blocked. The blocking step is performed to prevent “downward” nucleotide additions to the 3' end of the template during the sequencing process (details of the sequencing strategy and chemistry have been described previously (32)). Polyadenylated RNA is captured on the sequencing flow cell surfaces coated with poly(dT) oligonucleotides through <t>hybridization.</t> A “fill” step is performed with dTTP and polymerase, and then the templates are “locked” in position with fluorescently labeled proprietary Virtual Terminator™ (VT)-A, -C and -G sequencing nucleotide analogs. VT analogs are nucleotides used for sequencing, containing a fluores-cent dye and chemically cleavable groups that prevent the addition of another nucleotide. These “fill and lock” steps correct for any misalignments that may be present in poly-A/T duplexes, and ensure that the sequencing starts in the template rather than the poly-A tail.
2× Hybridization Buffer Helicos Rna Sequencing Kit, supplied by Helicos Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/2× hybridization buffer helicos rna sequencing kit/product/Helicos Inc
Average 90 stars, based on 1 article reviews
2× hybridization buffer helicos rna sequencing kit - by Bioz Stars, 2026-04
90/100 stars
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2× hybridization buffer  (Amersham Pharmacia Biotech Ltd)
90
Amersham Pharmacia Biotech Ltd 2× hybridization buffer
DRS sample preparation. RNA species which contain a 3' poly-A tail require 3' end blocking as described. Other RNA species are enzymatically 3' polyadenylated and 3' blocked. The blocking step is performed to prevent “downward” nucleotide additions to the 3' end of the template during the sequencing process (details of the sequencing strategy and chemistry have been described previously (32)). Polyadenylated RNA is captured on the sequencing flow cell surfaces coated with poly(dT) oligonucleotides through <t>hybridization.</t> A “fill” step is performed with dTTP and polymerase, and then the templates are “locked” in position with fluorescently labeled proprietary Virtual Terminator™ (VT)-A, -C and -G sequencing nucleotide analogs. VT analogs are nucleotides used for sequencing, containing a fluores-cent dye and chemically cleavable groups that prevent the addition of another nucleotide. These “fill and lock” steps correct for any misalignments that may be present in poly-A/T duplexes, and ensure that the sequencing starts in the template rather than the poly-A tail.
2× Hybridization Buffer, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/2× hybridization buffer/product/Amersham Pharmacia Biotech Ltd
Average 90 stars, based on 1 article reviews
2× hybridization buffer - by Bioz Stars, 2026-04
90/100 stars
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50% formamide in hybridization buffer (2 × sodium saline citrate (ssc)/50% formamide)  (PANAGENE Inc)
90
PANAGENE Inc 50% formamide in hybridization buffer (2 × sodium saline citrate (ssc)/50% formamide)
DRS sample preparation. RNA species which contain a 3' poly-A tail require 3' end blocking as described. Other RNA species are enzymatically 3' polyadenylated and 3' blocked. The blocking step is performed to prevent “downward” nucleotide additions to the 3' end of the template during the sequencing process (details of the sequencing strategy and chemistry have been described previously (32)). Polyadenylated RNA is captured on the sequencing flow cell surfaces coated with poly(dT) oligonucleotides through <t>hybridization.</t> A “fill” step is performed with dTTP and polymerase, and then the templates are “locked” in position with fluorescently labeled proprietary Virtual Terminator™ (VT)-A, -C and -G sequencing nucleotide analogs. VT analogs are nucleotides used for sequencing, containing a fluores-cent dye and chemically cleavable groups that prevent the addition of another nucleotide. These “fill and lock” steps correct for any misalignments that may be present in poly-A/T duplexes, and ensure that the sequencing starts in the template rather than the poly-A tail.
50% Formamide In Hybridization Buffer (2 × Sodium Saline Citrate (Ssc)/50% Formamide), supplied by PANAGENE Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/50% formamide in hybridization buffer (2 × sodium saline citrate (ssc)/50% formamide)/product/PANAGENE Inc
Average 90 stars, based on 1 article reviews
50% formamide in hybridization buffer (2 × sodium saline citrate (ssc)/50% formamide) - by Bioz Stars, 2026-04
90/100 stars
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2× enhanced hybridization buffer (expresshyb™  (Becton Dickinson)
90
Becton Dickinson 2× enhanced hybridization buffer (expresshyb™
DRS sample preparation. RNA species which contain a 3' poly-A tail require 3' end blocking as described. Other RNA species are enzymatically 3' polyadenylated and 3' blocked. The blocking step is performed to prevent “downward” nucleotide additions to the 3' end of the template during the sequencing process (details of the sequencing strategy and chemistry have been described previously (32)). Polyadenylated RNA is captured on the sequencing flow cell surfaces coated with poly(dT) oligonucleotides through <t>hybridization.</t> A “fill” step is performed with dTTP and polymerase, and then the templates are “locked” in position with fluorescently labeled proprietary Virtual Terminator™ (VT)-A, -C and -G sequencing nucleotide analogs. VT analogs are nucleotides used for sequencing, containing a fluores-cent dye and chemically cleavable groups that prevent the addition of another nucleotide. These “fill and lock” steps correct for any misalignments that may be present in poly-A/T duplexes, and ensure that the sequencing starts in the template rather than the poly-A tail.
2× Enhanced Hybridization Buffer (Expresshyb™, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/2× enhanced hybridization buffer (expresshyb™/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
2× enhanced hybridization buffer (expresshyb™ - by Bioz Stars, 2026-04
90/100 stars
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Image Search Results


DRS sample preparation. RNA species which contain a 3' poly-A tail require 3' end blocking as described. Other RNA species are enzymatically 3' polyadenylated and 3' blocked. The blocking step is performed to prevent “downward” nucleotide additions to the 3' end of the template during the sequencing process (details of the sequencing strategy and chemistry have been described previously (32)). Polyadenylated RNA is captured on the sequencing flow cell surfaces coated with poly(dT) oligonucleotides through hybridization. A “fill” step is performed with dTTP and polymerase, and then the templates are “locked” in position with fluorescently labeled proprietary Virtual Terminator™ (VT)-A, -C and -G sequencing nucleotide analogs. VT analogs are nucleotides used for sequencing, containing a fluores-cent dye and chemically cleavable groups that prevent the addition of another nucleotide. These “fill and lock” steps correct for any misalignments that may be present in poly-A/T duplexes, and ensure that the sequencing starts in the template rather than the poly-A tail.

Journal: Methods in Molecular Biology (Clifton, N.j.)

Article Title: Transcriptome Profiling Using Single-Molecule Direct RNA Sequencing

doi: 10.1007/978-1-61779-089-8_4

Figure Lengend Snippet: DRS sample preparation. RNA species which contain a 3' poly-A tail require 3' end blocking as described. Other RNA species are enzymatically 3' polyadenylated and 3' blocked. The blocking step is performed to prevent “downward” nucleotide additions to the 3' end of the template during the sequencing process (details of the sequencing strategy and chemistry have been described previously (32)). Polyadenylated RNA is captured on the sequencing flow cell surfaces coated with poly(dT) oligonucleotides through hybridization. A “fill” step is performed with dTTP and polymerase, and then the templates are “locked” in position with fluorescently labeled proprietary Virtual Terminator™ (VT)-A, -C and -G sequencing nucleotide analogs. VT analogs are nucleotides used for sequencing, containing a fluores-cent dye and chemically cleavable groups that prevent the addition of another nucleotide. These “fill and lock” steps correct for any misalignments that may be present in poly-A/T duplexes, and ensure that the sequencing starts in the template rather than the poly-A tail.

Article Snippet: The RNA samples are mixed 50:50 with 2× hybridization buffer provided in the Helicos RNA Sequencing Kit (Helicos BioSciences Corporation).

Techniques: Sample Prep, Blocking Assay, Sequencing, Hybridization, Labeling